Publications:
1. Sánchez JM. ;Lopez-Laguna H. ;Parladé E. ;Di Somma A. ;Livieri AL. ;Alamo P. ;Mangues R. ;Unzueta U. ;Villaverde A. ;Vazquez E. Structural Stabilization of Clinically Oriented Oligomeric Proteins During their Transit through Synthetic Secretory Amyloids. Advanced Science. 11:2309427. 2024
2. Virgili AC. ;Salazar J. ;Gallardo A. ;Lopez-Pousa A. ;Teres R. ;Bague S. ;Orellana R. ;Fumagalli C. ;Mangues R. ;Alba-Castellon L. ;Unzueta U. ;Casanova I. ;Sebio A. CXCR4 Expression as a Prognostic Biomarker in Soft Tissue Sarcomas. Diagnostics. 14:1195. 2024
3. Lopez-Laguna H. ;Tsimbouri PM. ;Jayawarna V. ;Rigou I. ;Serna N. ;Volta-Duran E. ;Unzueta U. ;Salmeron-Sanchez M. ;Vazquez E. ;Dalby MJ. ;Villaverde A. Hybrid Micro-/Nanoprotein Platform Provides Endocrine-like and Extracellular Matrix-like Cell Delivery of Growth Factors. ACS Applied Materials & Interfaces. 16:32930. 2024
4. Favaro M. ;Lopez-Laguna H. ;Volta-Duran E. ;Alba-Castellon L. ;Sanchez JM ;Casanova I. ;Unzueta U. ;Mangues R. ;Villaverde A. ;Vazquez E. Lyophilization of biomimetic amyloids preserves their regulatable, endocrine-like functions for nanoparticle release. Applied Materials Today. 39:102348. 2024
1. Lopez-Laguna H.; Rueda A.; Martinez-Torro C.; Sanchez-Alba L.; Carratalà JV.; Atienza-Garriga J.; Parladè E.; Sanchez JM.; Serna N.; Voltà-Durán E.; Ferrer-Miralles N.; Reverter D.; Mangues R.; Villaverde A.; Vazquez E.; Unzueta U. Biofabrication of Self-Assembling Covalent Protein Nanoparticles through Histidne-Templated Cysteine Coupling. ACS sustainable Chemistry & Eng. 11:4133. 2023
2. Voltà-Durán E.; Parlade R.; Serna N.; Villaverde A.; Vazquez E.; Unzueta U.; Endosomal scape for cell-targeted proteins. Going out after going in. Biotechnology advances. 63:108103, 2023.
3. Rueda A.;Mendoza I., Alba-Castellon L., Parladé E., Voltà-Durán E., Paez D., Aviño A., Eritja R., Vázquez E., Villaverde A., Mangues R., Unzueta U. Site-directed cysteine coupling of disulfide-containing non-antibody carrier proteins (THIOCAPs). Science China-Materials. 66: 4109 . 2023.
4. Serna N., Lopez-Laguna H., AceitunoP., Rojas-Peña M., Parlade E., Voltá-Durán E., Martinez-Torro C., Sanchez JM., Di Somma A., Carratala JV., Livieri A., Ferrer-Miralles N., Vazquez E., Unzueta U., Roher N., Villaverde A. Efficient Delivery of Antimicrobial Peptides in an Innovative, Slow-Release Pharmacological Formulation.Pharmacological Formulation. Pharmaceutics. 15: 2632. 2023.
5. Voltà-Durán E., Alba-Castellón L., Serna N., Casanova I., López-Laguna H., Gallardo A., Sánchez-Chardi A., Villaverde A., Unzueta U., Vázquez E., Mangues R. High-precision targeting and destruction of cancer-associated PDGFR-β+ stromal fibroblasts through self-assembling, protein-only nanoparticles. Acta Biomaterialia. 170:543. 2023.
6. Martínez-Torró C, Alba-Castellón L, Carrasco-Díaz LM, Serna N, Imedio L, Gallardo A, Casanova I, Unzueta U, Vázquez E, Mangues R, Villaverde A. Lymphocyte infiltration and antitumoral effect promoted by cytotoxic inflammatory proteins formulated as self-assembling, protein-only nanoparticles. Biomedicine & Pharmacotherapy. 164:114976. 2023.
7. Parladé E, Sánchez JM, López-Laguna H, Unzueta U, Villaverde A, Vázquez E. Protein features instruct the secretion dynamics from metal-supported synthetic amyloids. International Journal of Biological Macromolecules. 250:126164, 2023.
8. Núñez Y, Garcia-León A, Falgàs A, Serna N, Sánchez-García L, Garrido A, Sierra J, Gallardo A, Unzueta U, Vázquez E, Villaverde A, Mangues R, Casanova I. T22-PE24-H6 Nanotoxin Selectively Kills CXCR4-High Expressing AML Patient Cells In Vitro and Potently Blocks Dissemination In Vivo. Pharmaceutics, 15:727 . 2023
1. Unzueta U. ;Sanchez JM. ;Garcia-Fruitos E. ;Seras-Franzolo J. Editorial: High added-value nanoparticles: Rethinking and recycling cell protein waste. Frontiers in Bioengineering and Biotechnology.10:1018014. 2022. IF: 5,700, 1er Quartil
2. Cano-Garrido O. ;Serna N. ;Unzueta U. ;Parlade E. ;Mangues R. ;Villaverde A. ;Vazquez E. Protein scaffolds in human clinics. BIOTECHNOL ADV. 61: 108032. 2022.
3. Pallarès V. ;Unzueta U. ;Falgàs A. ;Aviño A. ;Nuñez Y. ;García-León A. ;Sanchez-Garcia L. ;Serna N. ;Gallardo A. ;Alba-Castellón L. ;Álamo P. ;Sierra J. ;Cedó L. ;Eritja R. ;Villaverde A. ;Vazquez E. ;Casanova I. ;Mangues R. A multivalent Ara-C-prodrug nanoconjugate achieves selective ablation of leukemic cells in an acute myeloid leukemia mouse model. BIOMATERIALS. 280: 121258. 2022.
4. Serna N. ;Pallares V. ;Unzueta U. ;Garcia-Leon A. ;Voltà-Durán E. ;Sanchez-Chardi A. ;Parlade E. ;Rueda A. ;Casanova I. ;Falgas A. ;Alba-Castellon L. ;Sierra J. ;Villaverde A. ;Vazquez E. ;Mangues R. Engineering non-antibody human proteins as efficient scaffolds for selective, receptor-targeted drug delivery. Journal of Controlled Release. 242: 277. 2022.
5. Lopez-Laguna H. ;Voltà-Duran E. ;Parlade E. ;Villaverde A. ;Vazquez E. ;Unzueta U. Insights on the emerging biotechnology of histidine-rich peptides. BIOTECHNOL ADV. 54: 107817. 2022.
6. Volta-Duran E. ;Sanchez JM. ;Parlade E. ;Serna N. ;Vazquez E. ;Unzueta U. ;Villaverde A. The Diphtheria Toxin Translocation Domain Impairs Receptor Selectivity in Cancer Cell-Targeted Protein Nanoparticles. Pharmaceutics. 14: 2644. 2022.
7. Rioja-Blanco E. ;Arooyo-Solera I. ;Alamo P. ;Casanova I. ;Gallardo A. ;Unzueta U. ;Serna N. ;Sanchez-Garcia L. ;Quer M. ;Villaverde A. ;Vazquez E. ;Mangues R. ;Alba-Castellon L. ;Leon X. Self-assembling protein nanocarrier for selective delivery of cytotoxic polypeptides to CXCR4+ head and neck squamous cell carcinoma tumors. Acta Pharmaceutica Sinica B. 12: 2578. 2022.
8. Falgás A. ;Garcia-León A. ;Núñez Y. ;Serna N. ;Sanchez-Garcia L. ;Unzueta U. ;Voltà-Durán E. ;Aragó M. ;Álamo P. ;Alba-Castellon L. ;Sierra J. ;Gallardo A. ;Villaverde A. ;Vazquez E. ;Mangues R. ;Casanova I. A diphtheria toxin-based nanoparticle achieves specific cytotoxic effect on CXCR4+ lymphoma cells without toxicity in immunocompromised and immunocompetent mica. Biomedicine & Pharmacotherapy. 150: 112940. 2022.
9. Rioja-Blanco E. ;Gallardo A. ;Arroyo-Solera I. ;Álamo P. ;Casanova I. ;Unzueta U. ;Serna N. ;Sanchez-Garcia L. ;Quer M. ;Villaverde A. ;Vazquez E. ;León X. ;Alba-Castellon L. ;Mangues R. A Novel CXCR4-Targeted Diphtheria Toxin Nanoparticle Inhibits Invasion and Metastatic Dissemination in a Head and Neck Squamous Cell Carcinoma Mouse Model. Pharmaceutics. 14: 887. 2022.
10. Parladé E. ;Voltà-Durán E. ;Cano-Garrido O. ;Sanchez JM. ;Unzueta U. ;Lopez-Laguna H. ;Serna N. ;Cano M. ;Rodriguez-Mariscal M. ;Vazquez E. ;Villaverde A. An In Silico Methodology That Facilitates Decision Making in the Engineering of Nanoscale Protein Materials. Int. J. of Molecular Science. 23: 4958. 2022.
11. Rioja-Blanco E. ;Arroyo-Solera I. ;Álamo A. ;Casanova I. ;Gallardo A. ;Unzueta U. ;Serna N. ;Sanchez-Garcia L. ;Quer M. ;Villaverde A. ;Vaquez E. ;León X. ;Alba-Castellon L. ;Mangues R. CXCR4-targeted nanotoxins induce GSDMEdependent pyroptosis in head and neck squamous cell carcinoma. J EXP CLIN CANC RES. 41:49. 2022.
12. Sala R. ;Rioja-Blanco E. ;Serna N. ;Sanchez-Garcia L. ;Alamo P. ;Alba-Castellon L. ;Casanova I. ;Lopez-Pousa A. ;Unzueta U. ;Cespedes MV. ;Vazquez E. ;Villaverde A. ;Mangues R. GSDMD-dependent pyroptotic induction by a multivalent CXCR4-targeted nanotoxin blocks colorectal cancer metàstasis. Drug Delivery. 29:1384. 2022.
13. Medina-Gutierrez E. ;Cespedes MV. ;Gallardo A. ;Rioja-Blanco E. ;Pavón MA. ;Asensio-Puig L. ;Farre L. ;Alba-Castellon L. ;Unzueta U. ;Villaverde A. ;Vazquez E. ;Casanova I. ;Mangues R. Novel Endometrial Cancer Models Using Sensitive Metastasis Tracing for CXCR4-Targeted Therapy in Advanced Disease. Biomedicines. 10: 1680. 2022.
14. Medina-Gutierrez E. ;Garcia-Leon A. ;Gallardo A. ;Alamo P. ;Alba-Castellon L. ;Unzueta U. ;Villaverde A. ;Vazquez E. ;Casanova I. ;Mangues R. Potent Anticancer Activity of CXCR4-Targeted Nanostructured Toxins in Aggressive Endometrial Cancer Models. Cancers. 15. 2022.
15. Sanchez JM. ;Carratalá JV. ;Serna N. ;Unzueta U. ;Nolan V. ;Sanchez-Chardi A. ;Voltà-Durán E. ;Lopez-Laguna H. ;Ferrer-Miralles N. ;Villaverde A. ;Vazquez E. The Poly-Histidine Tag H6 Mediates Structural and Functional Properties of Disintegrating, Protein-Releasing Inclusion Bodies. Phamaceutics. 14:602. 2022.
16. Voltà-Durán E. ;Sanchez JM. ;Lopez-Laguna H. ;Parlade E. ;Sanchez-Garcia L. ;Sanchez-Chardi A. ;De Marco A. ;Unzueta U. ;Vazquez E. ;Villaverde A. The spectrum of building block conformers sustains the biophysical properties of clinically-oriented self-assembling protein nanoparticles. SCIENCE CHINA Materials. 65:1662. 2022.
17. Serna N. ;Falgàs A. ;Garcia-León A. ;Unzueta U. ;Nuñez Y. ;Sanchez-Chardi A. ;Martinez-Torro C. ;Mangues R. ;Vazquez E. ;Casanova I. ;Villaverde A. Time-Prolonged Release of Tumor-Targeted Protein–MMAE Nanoconjugates from Implantable Hybrid Materials. Pharmaceutics. 14:192. 2022.
18. Barguilla I. ;Unzueta U. ;Carratalá JV. ;Cano-Garrido O. ;Villaverde A. ;Hernandez A. ;Ferrer-Miralles N. Toxicity Profiling of Bacterial Inclusion Bodies in Human Caco-2 Cells. Frontiers in Bioengineering and Biotechnology. 10: 842256. 2022.
1. Lopez-Laguna H. ;Sanchez JM. ;Carratalá JV. ;Rojas-Peña M. ;Sanchez-Garcia L. ;Parladé E. ;Sanchez-Chardi A. ;Voltà-Durán E. ;Serna N. ;Cano-Garrido O. ;Flores S. ;Ferrer-Miralles N. ;Nolan V. ;de Marco A. ;Roher N. ;Unzueta U. ;Vazquez E. ;Villaverde A. Biofabrication of functional protein nanoparticles through simple His-tag engineering. ACS SUSTAIN CHEM ENG. 9, 1241 – 12354. 2021.
2. Lopez-Laguna H. ;Parladé E. ;Álamo P. ;Sánchez JM. ;Voltà-Durán E. ;Serna N. ;Sanchez-Garcia L. ;Cano-Garrido O. ;Sanchez-Chardi A. ;Villaverde A. ;Mangues R. ;Unzueta U. ;Vazquez E. In Vitro Fabrication of Microscale Secretory Granules. ADV FUNCT MATER. 2100914. 2021
3. Alamo P. ;Cedano J. ;Conchillo-Sole O. ;Cano-Garrido O. ;Alba-Casterllon L. ;Serna S. ;Aviño A. ;Carrasco-Dias LM. ;Sanchez-Chardi A. ;Martinez-Torro C. ;Gallardo A. ;Cano M. ;Eritja R. ;Villaverde A. ;Mangues R. ;Vazquez E. ;Unzueta U. Rational engineering of a human GFP-like protein scaffold for humanized targeted nanomedicines. ACTA BIOMATER. 130, 211. 2021
4. Volta-Duran E. ;Serna N. ;Sanchez-Garcia L. ;Aviño A. ;Sanchez JM. ;Lopez-Laguna H. ;Cano-Garrido O. ;Casanova I. ;Mangues R. ;Eritja R. ;Vazquez E. ;Villaverde A. ;Unzueta U. Design and engineering of tumor-targeted, dual-acting cytotoxic nanoparticles. ACTA BIOMATER. 119, 312-322. 2021
5. Serna N. ;Carratalá JV. ;Conchillo-Sole O. ;Martinez-Torro C. ;Unzueta U. ;Mangues R. ;Ferrer-Miralles N. ;Daura X. ;Vazquez E. ;Villaverde A. Antibacterial Activity of T22, a Specific Peptidic Ligand of the Tumoral Marker CXCR4. PHARMACEUTICS. 13, 1922. 2021.
6. Alamo P. ;Parlade E. ;Lopez-Laguna H. ;Volta-Duran E. ;Unzueta U. ;Vazquez E. ;Mangues R. ;Villaverde A. Ion-dependent slow protein release from in vivo disintegrating micro-granules.DRUG DELIVERY. 28, 2383 – 2391. 2021.
7. Sanchez-Garcia L. ;Voltà-Durán E. ;Parladé E. ;Mazzega E. ;Sanchez-Chardi A. ;Serna N. ;Lopez-Laguna H. ;Mitstorfer M. ;Unzueta U. ;Vazquez E. ;Villaverde A. ;de Marco A. Self-Assembled Nanobodies as Selectively Targeted, Nanostructured, and Multivalent Materials. ACS APPL MATER INTER. 13, 29406 – 29415. 2021.
8. Cano-Garrido O. ;Alamo P. ;Sanchez-Garcia L. ;Falgas A. ;Sanchez-Chardi A. ;Serna N. ;Parlade E. ;Unzueta U. ;Roldan M. ;Volta-Duran E. ;Casanova I. ;Villaverde A. ;Mangues R. ;Vazquez E. Biparatopic Protein Nanoparticles for the Precision Therapy of CXCR4+ Cancers. CANCERS. 13, 2929. 2021.
9. Pallares V. ;Nuñez Y. ;Sanchez-Garcia L. ;Falgas A. ;Serna N. ;Unzueta U. ;Gallardo A. ;Alba-Castellon L. ;Alamo P. ;Sierra J. ;Villaverde A. ;Vazquez E. ;Casanova I. ;Mangues R. Antineoplastic effect of a diphtheria toxin-based nanoparticle targeting acute myeloid leukemia cells overexpressing CXCR4. J CONTROL RELEASE. 335, 117 – 129. 2021.
10. Sanchez JM. ;Lopez-Laguna H. ;Serna N. ;Unzueta U. ;Clop D. ;Villaverde A. ;Vazquez E. Engineering the Performance of Artificial Inclusion Bodies Built of Catalytic β‑Galactosidase. ACS SUSTAIN CHEM ENG, 9, 2552-2558. 2021.
11. Falgàs A. ;Pallares V. ;Unzueta U. ;Nuñez Y. ;Sierra J. ;Gallardo A. ;Alba-Castellon L. ;Mangues MA. ;Alamo P. ;Villaverde A. ;Vazquez E. ;Mangues R. ;Casanova I. Specific Cytotoxic Effect of an Auristatin Nanoconjugate Towards CXCR4+ Diffuse Large B-Cell Lymphoma Cells. INT J NANOMED, 16, 1869-1888. 2021.
1. Voltà-Durán E. ;Cano-Garrido O. ;Serna N. ;Lopez-Laguna H. ;Sanchez-Garcia L. ;Pesarrodona M. ;Sanchez-Chardi A. ;Mangues R. ;Villaverde A. ;Vazquez E. ;Unzueta U. Controlling self-assembling and tumor celltargeting of protein-only nanoparticles through modular protein engineering. SCI CHINA MATER. 63, 147-156. 2020.
2. Unzueta U. ;Roldan M. ;Pesarrodona M. ;Benitez R. ;Sanchez-Chardi A. ;Conchillo-Sole O. ;Mangues R. ;Villaverde A. ;Vazquez E. Self-assembling as regular nanoparticles dramatically minimizes photobleaching of tumor-targeted GFP. ACTA BIOMATER. 103, 272-280. 2020
3. López-Laguna H. ;Cubarsi R. ;Unzueta U. ;Mangues R. ;Vazquez E. ;Villaverde A. Endosomal escape of protein nanoparticles engineered through humanized histidine-rich peptides. SCI CHINA MATER. 63, 644-653. 2020.
4. Pallares V. ;Unzueta U. ;Falgàs A. ;Sanchez-Garcia L. ;Serna N. ;Gallardo A. ;Morris GA. ;Alba-Castellon L. ;Alamo P. ;Sierra J. ;Villaverde A. ;Vazquez E. ;Casanova I. ;Mangues R. An Auristatin nanoconjugate targeting CXCR4+ leukemic cells blocks acute myeloid leukemia dissemination. J HEMATOL ONCOL. 13-36. 2020.
5.Lopez-Laguna H. ;Sanchez-Garcia L. ;Serna N. ;Voltà-Durán E. ;Sanchez JM. ;Sanchez-chardi A. ;Unzueta U. ;Los M. ;Villaverde A. ;Vazquez E. Engineering Protein Nanoparticles Out From Components of the Human Microbiome. SMALL. 16, e2001885. 2020.
6. Lopez-Laguna H. ;Sanchez JM. ;Unzueta U. ;Mangues R. ;Vazquez E. ;Villaverde A. Divalent Cations: A Molecular Glue for Protein Materials. TRENDS BIOCHEM SCI. 45-11, 992-1003. 2020.
7. Alamo P. ;Pallarès V. ;Cespedes MV. ;Falgas A. ;Sanchez JM. ;Serna N. ;Sanchez-Garcia L. ;Voltà-Durán E. ;Morria GA. ;Sanchez-Chardi A. ;Casanova I. ;Mangues R. ;Vazquez E. ;Villaverde A. ;Unzueta U. Fluorescent Dye Labeling Changes the Biodistribution of Tumor-Targeted Nanoparticles. PHARMACEUTICS. 22-11, E1004. 2020.
8. Falgàs A. ;Pallarès V. ;Unzueta U. ;Cespedes MV. ;Moreno MJ. ;Gallardo A. ;Mangues MA. ;Sierra J. ;Villaverde A. ;Vazquez E. ;Mangues R. ;Casanova I. A CXCR4-targeted nanocarrier achieves highly selective tumor uptake in diffuse large B-cell lymphoma mouse models. HAEMATOLOGICA, 105:3, 741-753. 2020.
9. Cespedes MV. ;Cano-Garrido O. ;Alamo P. ;Rita S. ;Gallardo A. ;Serna N. ;Falgàs A. ;Voltà-Durán E. ;Casanova I. ;Sanchez-chardi A. ;Lopez-Laguna H. ;Sanchez-Garcia L. ;Sanchez JM. ;Unzueta U. ;Vazquez E. ;Mangues R. ;Villaverde A. Engineering Secretory Amyloids for Remote and Highly Selective Destruction of Metastatic Foci. ADV. MATER. 32, 1907348. 2020.
10. Serna N. ;Alamo P. ;Ramesh P. ;Vinokurova D. ;Sanchez-Garcia L. ;Unzueta U. ;Gallardo A. ;Cespedes MV. ;Vazquez E. ;Villaverde A. ;Mangues R. ;Medema JP. Nanostructured toxins for the selective destruction of drug resistant human CXCR4+ colorectal cancer stem cells. J CONTROL RELEASE. 320, 96-104. 2020.
11. Falgàs A. ;Pallares V. ;Serna N. ;Sanchez-Garcia L. ;Sierra J. ;Gallardo A. ;Alba-Castellon L. ;Alamo P. ;Unzueta U. ;Villaverde A. ;Vazquez E. ;Mangues R. ;Casanova I. Selective Delivery of T22-PE24-H6 to CXCR4 + Diffuse Large B-cell Lymphoma Cells Leads to Wide Therapeutic Index in a Disseminated Mouse Model. THERANOSTICS. 6-12, 5169-5180. 2020.
12. Serna N. ;Cano-Garrido O. ;Sanchez-Garcia L. ;Pesarrodona M. ;Unzueta U. ;Sanchez-Chardi A. ;Mangues R. ;Vazquez E. ;Villaverde A. Engineering Protein Venoms as Self-Assembling CXCR4-Targeted Cytotoxic Nanoparticles. PART PART SYST CHAR. 2000400. 2020.
13. Sanchez-Garcia L. ;Sala R. ;Serna N. ;Álamo P. ;Parladé E. ;Alta-Castellón L. ;Voltà-Durán E. ;Sanchez-Chardi A. ;Unzueta U. ;Vazquez E. ;Mangues R. ;Villaverde A. A refined cocktailing of pro-apoptotic nanoparticles boosts anti-tumor activity. ACTA BIOMATER. 113, 584-596. 2020.
14. Serna N. ;Carratalá JV. ;Parladé E. ;Sanchez-Chardi A. ;Aviño A. ;Unzueta U. ;Mangues R. ;Eritja R. ;Ferrer-Miralles N. ;Vazquez E. ;Villaverde A. Developing Protein−Antitumoral Drug Nanoconjugates as Bifunctional Antimicrobial Agents. ACS APPL MATER INTER. 12-52, 57746-57756. 2020.
1. López-Laguna H. ;Unzueta U. ;Conchillo-Solé O. ;Sanchez-Chardi A. ;Pesarrodona M. ;Cano-Garrido O. ;Voltà E. ;Sánchez-García L. ;Serna N. ;Saccardo P. ;Mangues R. ;Villaverde A. ;Vazquez E. Assembly of histidine-rich protein materials controlled through divalent cations. ACTA BIOMATER. 83, 257-264. 2019.
2. Diaz R. ;Sanchez-Garcia L. ;Serna N. ;Sanchez-chardi A. ;Cano-Garrido O. ;Sanchez JM. ;Unzueta U. ;Vazquez E. ;Villaverde A. Engineering a recombinant chlorotoxin as cell-targeted cytotoxic nanoparticles. SCI CHINA MATER. 62-6, 892-898. 2019.
3. Casanova I. ;Unzueta U. ;Arroyo-Solera I. ;Cespedes MV. ;Villaverde A. ;Mangues R. ;Vazquez E. Protein-driven nanomedicines in oncotherapy. CURR OPIN PHARMACOL. 47, 1-7. 2019.
4. Lopez-Laguna H. ;Sala R. ;Sanchez JM. ;Alamo P. ;Unzueta U. ;Sanchez-Chardi A. ;Serna N. ;Sanchez-Garcia L. ;Voltà-Durán E. ;Mangues R. ;Villaverde A. ;Vazquez E. Nanostructure empowers active tumor targeting in ligand-based molecular delivery. PART PART SYST CHAR. 36, 1900304. 2019.
5. Aviño A. ;Unzueta U. ;Cespedes MV. ;Casanova I. ;Vazquez E. ;Villaverde A. ;Mangues R. ;Eritja R. Efficient bioactive oligonucleotide-protein conjugation for cell-targeted cancer therapy. CHEMISTRYOPEN. 8, 382-387. 2019.
6. Serna N. ;Sanchez JM ;Unzueta U. ;Sanchez-Garcia L. ;Sanchez-Chardi A. ;Manguez R. ;Vazquez E. ;Villaverde A. Recruiting potent membrane penetrability in tumor cell-targeted protein-only nanoparticles. NANOTECHNOLOGY 30-11, 115101. 2019.
7. Sala R. ;Sánchez-García L. ;Serna N. ;Céspedes MV ;Casanova I. ;Roldán M. ;Sáncez-chardi A. ;Unzueta U. ;Vazquez E. ;Mangues R. ;Villaverde A. Collaborative membrane activity and receptor-dependent tumor cell targeting for precise nanoparticle delivery in CXCR4+ colorectal cancer. ACTA BIOMATER. 99, 426-432. 2019.
8. Sánchez JM. ;López-Laguna H. ;Álamo P. ;Serna N. ;Sánchez-Chardi A. ;Nolan V. ;Cano-Garrido O. ;Casanova I. ;Unzueta U. ;Vazquez E. ;Mangues R. ;Villaverde A. Artificial Inclusion Bodies for Clinical Development. ADV SCI. 7-3, 1902420. 2019.
1. Unzueta U. ;Cespedes MV. ;Sala R. ;Alamo P. ;Sanchez-Chardi A. ;Pesarrodona M. ;Sanchez-Garcia L. ;Cano-Garrido O. ;Villaverde A. ;Vazquez E. ;Mangues R. ;Seras-Franzoso J. Release of targeted protein nanoparticles from functional bacterial amyloids: A death star-like approach. J CONTROL RELEASE. 279, 29-39. 2018.
2. Cespedes MV. ;Unzueta U. ;Aviño A. ;Gallardo A. ;Alamo P. ;Sala R. ;Sanchez-Chardi A. ;Casanova I. ;Magues MA ;Lopez-Pousa A. ;Eritja R. ;Villaverde A. ;Vazquez E. ;Mangues R. Selective depletion of metastatic stem cells as therapy for human colorectal cancer. EMBO MOL MED. 10-10, e8772. 2018.
3. Favaro MTP. ;Unzueta U. ;de Cabo M. ;Villaverder A. ;Ferrer-Miralles N. ;Azzoni AR. Intracellular trafficking of a dynein-based nanoparticle designed for gene delivery. EUR J PHARM SCI. 112, 71-78. 2018.
4. De Pinho Favaro MT ;Sanchez-Garcia L. ;Sanchez-Chardi A. ;Roldan M. ;Unzueta U. ;Serna N. ;Cano-Garrido O. ;Azzoni AR. ;Ferrer-Miralles N. ;Villaverde A. ;Vazquez E. Protein nanoparticles are nontoxic, tuneable cell stressors. NANOMEDICINE-UK. 13-3, 255-268. 2018.
5. Sanchez-Garcia L. ;Serna N. ;Alamo P. ;Sala R. ;Cespedes MV. ;Roldan M. ;Sanchez-chardi A. ;Unzueta U. ;Casanova I. ;Mangues R. ;Vazquez E. ;Villaverde A. Self-assembling toxin-based nanoparticles as self-delivered antitumoral drugs. J CONTROL RELEASE. 274, 81-92. 2018.
6. Diaz R. ;Pallares V. ;Cano-Garrido O. ;Serna N. ;Sanchez-Garcia L. ;Falgas A. ;Pesarrodona M. ;Unzueta U. ;Sanzhez-Chardi A. ;Sanchez JM. ;Casanova I. ;Vazquez E. ;Magues R. ;Villaverde A. Selective CXCR4+ Cancer Cell Targeting and Potent Antineoplastic Effect by a Nanostructured Version of Recombinant Ricin. SMALL. 14-26, e1800665. 2018.
7. De Pinho Favaro MT. ;Serna N. ;Sanchez-Garcia L. ;Cubarsi R. ;Roldan M. ;Sanchez-Chardi A. ;Unzueta U. ;Mangues R. ;Ferrer-Miralles N. ;Azzoni AR. ;Vazquez E. ;Villaverde A. Switching cell penetrating and CXCR4-binding activities of nanoscale-organized arginine-rich peptides. NANOMED-NANOTECHNOL. 14-6, 1777-1786. 2018.
8. Sanchez MJ. ;Sanchez-Garcia L. ;Pesarrodona M. ;Serna N. ;Sanchez-Chardi A. ;Unzueta U. ;Mangues R. ;Vazquez E. ;Villaverde A. Conformational Conversion during Controlled Oligomerization into Nonamylogenic Protein Nanoparticles. BIOMACROMOLECULES. 19-9, 3788-3797. 2018.
9. Serna N. ;Sanchez-Garcia L. ;Unzueta U. ;Diaz R. ;Vazquez E. ;Mangues R. ;Villaverde A. Protein-based therapeutic killing for cancer therapies. TRENDS BIOTECHNOL. 36-3, 318-335. 2018.
1. Unzueta U. ;Seras-Franzoso J. ;Cespedes MV. ;Saccardo P. ;Cortes F. ;Rueda F. ;Garcia-Fruitos E. ;Ferrer-Miralles N. ;Magues R. ;Vazquez E. ;Villaverde A. Engineering tumor cell targeting in nanoscale amyloidal materials. NANOTECHNOLOGY. 28-1, 015102. 2017.
2. Sanchez-Garcia L. ;Serna N. ;Mattanovich M. ;Cazzanelli P. ;Sanchez-Chardi A. ;Conchillo-sole O. ;Cortes F. ;Daura X. ;Unzueta U. ;Mangues R. ;Villaverde A. ;Vazquez E. The fusogenic peptide HA2 impairs selectivity of CXCR4-targeted protein nanoparticles. CHEM COMMUN. 53-33, 4565-4568. 2017.
3. Unzueta U. ;Serna N. ;Sanchez-Garcia L. ;Roldan M. ;Sanches-Chardi A. ;Mangues R. ;Villaverde A. ;Vazquez E. Engineering multifunctional protein nanoparticles by in vitro disassembling and reassembling of heterologous building blocs. NANOTECHNOLOGY. 28-50, 505102. 2017.
4. Pesarrodona M. ;Crosas E. ;Cubarsi R. ;Sanchez-Chardi A. ;Saccardo P. ;Unzueta U. ;Rueda F. ;Sanchez-Garcia L. ;Serna N. ;Mangues R. ;Ferrer-Miralles N. ;Vazquez E. ;Villaverde A. Intrinsic functional and architectonic heterogeneity of tumor-targeted protein nanoparticles. NANOSCALE. 9, 6427-6435. 2017.
5. Serna N. ;Cespedes MV. ;Sanchez-Garcia L. ;Unzueta Elorza, Ugutz ;Sala R. ;Sanchez-Chardi A. ;Cortes F. ;Ferrer-Miralles N. ;Mangues R. ;Vazquez E. ;villaverde A. Peptide-based nanostructured materials with intrinsic pro-apoptotic activities in CXCR4+ solid tumors. ADV FUNCT MATER. 27, 1700919. 2017.
6. Serna N. ;Sanchez-Garcia L. ;Sanchez-Chardi A. ;Unzueta U. ;Roldan M. ;Mangues R. ;Vazquez E. ;Villaverde A. Protein-only, antimicrobial peptide-containing recombinant nanoparticles with inherent built-in antibacterial activity. ACTA BIOMATER. 15-60, 256-263. 2017.
1. Serna N. ;Cespedes MV. ;Saccardo P. ;Xu Z. ;Unzueta U. ;Alamo P. ;Pesarrodona M. ;Sanchez-Chardi A. ;Roldan M. ;Mangues R. ;Vazquez E. ;Villaverde A. ;Ferrer-Miralles N. Rational engineering of single-chain polypeptides into protein-only, BBB-targeted nanoparticles. NANOMED-NANOTECHNOL. 12-5, 1241-1251. 2016.
2. Rueda F. ;Cespedes MV. ;Sanchez-Chardi A. ;Seras-Franzoso J. ;Pesarrodona M. ;Ferrer-Miralles N. ;Vazquez E. ;Rinas U. ;Unzueta U. ;Mammat U. ;Mangues R. ;Garcia-Fruitos E. ;Villaverde A. Structural and functional features of self-assembling protein nanoparticles produced in endotoxin-free Escherichia coli. MICROB CELL FACT. 15-1, 59. 2016.
3. Pesarrodona M. ;Fernandez Y. ;Foradada L. ;Sanchez-Chardi A. ;Conchillo-sole O. ;Unzueta U. ;Xu Z. ;Roldan M. ;Villegas S. ;Ferrer-Miralles N. ;Schwartz S. ;Rinas U. ;Daura X. ;Abasolo I. ;Vazquez E. ;Villaverde A. Conformational and functional variants of CD44-targeted protein nanoparticles bio-produced in bacteria. BIOFABRICATION. 8-2, 025001. 2016.
4. Cespedes MV. ;Unzueta U. ;Alamo P. ;Gallardo A. ;Sala R. ;Casanova I. ;Pavon MA. ;Mangues MA. ;Trias M. ;Lopez-Pousa A. ;Villaverde A. ;Vazquez E. ;Mangues R. Cancer-specific uptake of a liganded protein nanocarrier targeting aggressive CXCR4+ colorectal cancer models. NANOMED-NANOTECHNOL. 12-7, 1987-1996. 2016.
5. Cano-Garrido O. ;Céspedes MV. ;Unzueta U. ;Saccardo P. ;Roldan M. ;Sanchez-Chardi A. ;Curbasi R. ;Vazquez E. ;Mangues R. ;Garcia-Fruitos E. ;Villaverde A. CXCR4(+)-targeted protein nanoparticles produced in the food-grade bacterium Lactococcus lactis. NANOMEDICINE-UK. 11-18, 2387-2398. 2016.
6. Rueda F. ;Gasser B. ;Sanchez-Chardi A. ;Roldan M. ;Puxbaum V. ;Ferrer-Miralles N. ;Unzueta U. ;Vazquez E. ;Garcia-Fruitos E. ;Mattanovich D. ;Villaverde A. Functional inclusion bodies produced in the yeast Pichia pastoris. MICROB CELL FACT. 15-1, 166. 2016.
7. Céspedes MV. ;Fernandez Y. ;Unzueta U. ;Mendoza R. ;Seras-Franzoso J. ;Sanchez-Chardi A. ;Alamo P. ;Toledo-Rubio V. ;Ferrer-Miralles N. ;Vazquez E. ;Shwartz S. ;Abasolo I. ;Corchero JL. ;Mangues R. ;Villaverde A. Bacterial mimetics of endocrine secretory granules as immobilized in vivo depots for functional protein drugs. SCI REP-UK. 24-6, 35765. 2016.
1. Pesarrodona M. ;Unzueta U. ;Vazquez, E. Dialysis: a characterization method of aggregation tendency. Methods in Molecular Biology. 321 – 330. 2015.
2. Unzueta U. ;Vazquez E.. ;Accardi G. ;Mendoza R. ;Toledo-Rubio V. ;Giuliani M. ;Sannino F. ;Parrilli E. ;Abasolo I. ;Schwartz S Jr. ;Tutino ML. ;Villaverde A. ;Corchero JL. ;Ferrer-Miralles N. Strategies for the production of difficult-to-express full-length eukaryotic proteins using microbial cell factories: production of human alpha-galactosidase A. APPL MICROBIOL BIOT. 99:5863. 2015.
3. Peluffo H. ;Unzueta U. ;Negro-Demontel ML. ;Xu Z. ;Vazquez E. ;Ferrer-Miralles N. ;Villaverde A. BBB-targeting, protein-based nanomedicines for drug and nucleic acid delivery to the CNS. BIOTECHNOL ADV. 33:277. 2015.
4. Unzueta U.;Cespedes MV. ;Vazquez E. ;Ferrer-Miralles N. ;Mangues R. ;Villaverde A. Towards protein-based viral mimetics for cancer therapies. TRENDS BIOTECHNOL. 33: 253. 2015.
5. Rueda F. ;Cespedes MV. ;Conchillo-Sole O. ;Sanchez-Chardi A. ;Seras-Franzoso J. ;Curbasi R. ;Gallardo A. ;Pesarrodona M. ;Ferrer-Miralles N. ;Daura X. ;Vazquez E. ;Garcia-Fruitos E. ;Mangues R. ;Unzueta U. ;Villaverde A. Bottom-Up Instructive Quality Control in the Biofabrication of Smart Protein Materials. ADVANCED MATERIALS. 27:7816. 2015.
6. Zhikun X. ;Unzueta U. ;Roldan M. ;Mangues R. ;Sanchez-Chardi A. ;Ferrer-Miralles N. ;Villaverde A. ;Vazquez E. Formulating tumor-homing peptides as regular nanoparticles enhances receptor-mediated cell penetrability. MATERIAL LETTERS. 154:140. 2015.
7. Zhikun X. ;Cespedes MV. ;Unzueta U. ;Alamo P. ;Pesarrodona M. ;Mangues R. ;Vazquez E. ;Villaverde A. ;Ferrer-Miralles N. Targeting low-density lipoprotein receptors with protein-only nanoparticles. 17:150. 2015.
1. Unzueta u. ;Saccardo P. ;Domingo-Espin J. ;Cedano J. ;Conchillo-Sole O. ;Garcia-Fruitos E. ;Cespedes MV ;Corchero JL. ;Daura X. ;Mangues R. ;Ferrer-Miralles N. ;Villaverde A. ;Vazquez E. Sheltering DNA in self-organizing, protein-only nano-shells as artificial viruses for gene delivery. NANOMED-NANOTECHNOL. 10:535. 2014.
2. Céspedes MV ;Unzueta U. ;Tatkiewicz W ;Sánchez-Chardi A ;Conchillo-Solé O ;Alamo P ;Xu Z ;Casanova I ;Corchero JL ;Pesarrodona M ;Cedano J ;Daura X ;Ratera I ;Veciana J ;Ferrer-Miralles N ;Vazquez E ;Villaverde A ;Mangues R. In Vivo Architectonic Stability of Fully de Novo Designed Protein-Only Nanoparticles. ACS NANO. 8: 4166. 2014.
3. Pesarrodona, M. ;Ferrer-Miralles, N. ;Unzueta u. ;Gener P. ;Tatkiewicz W. ;Abasolo, I. ;Ratera, I. ;Veciana, J. ;Jr SS. ;Villaverde A. ;Vazquez, E. Intracellular targeting of CD44+ cells with self-assembling, protein only nanoparticles. INT J PHARMACEUT. 473:286. 2014.
1. Seras-Franzoso J ;Peebo K ;Corchero JL ;Tsimbouri P.M. ;Unzueta U ;Rinas U ;Dalby MJ ;Vazquez E ;García-Fruitós E ;Villaverde A. A nanostructured bacterial bio-scaffold for the sustained bottom-up delivery of protein drugs. NANOMEDICINE-UK. 8:1587. 2013.
2. Seras-Franzoso, J. ;Tsimbouri PM. ;Burgess KV. ;Unzueta u. ;Garcia-Fruitos, E. ;Vazquez E. ;Villaverde A. ;Dalby MJ. Topographically targeted osteogenesis of mesenchymal stem cells stimulated by inclusion bodies attached to polycaprolactone surfaces. NANOMEDICINE-UK. 9:207. 2013.
3. Ventosa N ;Cabrera I ;Elizondo E ;Esteban O ;Corchero JL ;Mergarejo M ;Pulido D ;Cordoba A ;Moreno-Calvo E ;Unzueta U ;Vazquez E ;Abasolo I ;Schwartz S ;Villaverde A ;Albericio F ;Royo M ;Garcia-Parajo M ;Veciana J. Multifunctional nanovesicle-bioactive conjugates prepared by a one-step scalable method using CO2-expanded solvents. NANO LETTER. 13:3766. 2013.
4. Unzueta U. ;Saccardo P ;Ferrer-Miralles N ;Cortés F ;García-Fruitós E ;Mangues R ;Vazquez E ;Villaverde A. Improved performance of protein-based recombinant gene therapy vehicles by tuning downstream procedures. BIOTECHNOL PROGR. 29:1458. 2013.
1. Unzueta U. ; Céspedes MV.; Ne Ferrer-Miralles N.; Casanova I. ; Cedano J. ; CorcheroJL. ; Domingo-Espín J. ; Villaverde A. ; Mangues R. ; Vázquez E. Intracellular CXCR4+ cell targeting with T22-empowered protein-only nanoparticles. INT J NANOMED. 7:4533 . 2012
2. Domingo-Espín J. ; Petegnief V.; de Vera N. ;Conchillo-Solé O. ; Saccardo P.; Unzueta U. ; Vazquez E. ; Cedano J. ; Negro L. ; Daura X. ; Peluffo H. ;. Planas A. ;Villaverde A. ; Ferrer-Miralles N. RGD-based cell ligands for cell-targeted drug delivery act as potent trophic factors. 8:1263. 2012.
3. Unzueta U. ;Ferrer-Miralles N ;Cedano J ;Zikung X ;Pesarrodona M ;Saccardo P ;García-Fruitós E ;Domingo-Espín J ;Kumar P ;Gupta KC ;Mangues R ;Villaverde A ;Vazquez E. Non-amyloidogenic peptide tags for the regulatable self-assembling of protein-only nanoparticles. BIOMATERIALS. 33:8714. 2012.
1. Joan Domingo-Espín ;Esther Vazquez ;Javier Ganz ;Oscar Conchillo ;Elena García-Fruitós ;Juan Cedano ;Ugutz Unzueta ;Valérie Petegnief ;Nuria Gonzalez-Montalbán ;Anna M Planas ;Xavier Daura ;Hugo Peluffo ;Neus Ferrer-Miralles ;Antonio Villaverde. Nanoparticulate architecture of protein-based artificial viruses is supported by protein-DNA interactions. NANOMEDICINE-UK. 6: 1047. 2011.
2. Joan Domingo Espín ;Ugutz Unzueta ;Paolo Saccardo ;Escarlata Rodríguez-Carmona ;Jose Luis Corchero ;Esther Vazquez ;Neus Ferrer-Miralles. Biological Entities for Drug Delivery and Gene Therapy: Protein Nanoparticles. PROG MOL BIOL TRANSL. 104:247. 2011